The Annafib project

Antimicrobial Nano-Functionalization of Peptide-enriched Silk Fibroinm atrices to prevent bone infections and to enhance implant osseointegration in orthopaedics and dentistry

Bacterial biofilm is a leading cause of infections in orthopaedics and dental implantology. Current antibiotic prophylaxis or therapy is inadequate to prevent biofilm formation and generates antibiotic resistance. The multidisciplinary approach of ANNAFIB combines tissue engineering and antimicrobial strategies to develop new products based on antimicrobial nano-functionalized silk fibroin-based scaffolds (ANF-PSF) to treat bone and/or periodontal infections.

The ANF-PSF local delivery of antibiotics with controlled release profiles and the modification of implant devices with osteoinductive properties represent an innovative
and synergistic approach to promote the bone deposition and to impede the biofilm formation in a one-step surgery. The ANNAFIB strategy will be validated through a translational approach in preclinical models.
The ANNAFIB products are expected to enhance bone and periprosthetic tissue regeneration together with antibacterial activities, as a novel therapeutic tool for biofilmrelated infections in orthopaedics (osteomyelitis and prosthetic joint infections) and dentistry (chronic periodontitis and septic implant loosening).

The project is coordinated by IRCCS Galeazzi Orthopedic Institute

The IRCCS Galeazzi Orthopedic Institute in Milan is a center of excellence for the research and treatment of musculoskeletal disorders.
Accredited with the Health System, it represents a center of excellence in clinical and pre-clinical biomedical research and in teaching as a teaching venue for the Degree Course in Medicine and Surgery. It is recognized as a Scientific Hospitalization and Treatment Institute (IRCCS) awarded by the Ministry of Health in the discipline of diseases of the musculoskeletal system and the acquisition of certification according to the UNI EN ISO 9001 standard for health areas and research management Scientific.